Platform for Animal Models and Experimental Surgery
La Animal Models and Experimental Surgery Platform of the ibs.GRANADA, with registration number ES180870000163 As a breeding center, supplier and user of experimental animals, it offers research and teaching staff the infrastructure, equipment and advice necessary for the development of preclinical research based on animal models.
Its fundamental objective is to guarantee the highest level of animal welfare and the correct application of the 3Rs Principle (Replacement, Reduction and Refinement), through strict compliance with current Spanish legislation (RD 53/2013, after its modification by RD 1083/2025) and European regulations (Directive 2010/63/EU).
All technical staff at the Platform have the professional training required by Order ECC/566/2015, which regulates the training necessary to work with experimental animals.
The Platform is used for biomedical research projects, teaching activities, and the development of surgical or therapeutic techniques involving the use of animals. All procedures, both experimental and educational, are reviewed beforehand by the Animal Experimentation Ethics Committee (CEEA) of ibs.GRANADA.
Furthermore, the ibs.GRANADA is part of the COSCE Agreement for Transparency in Animal Experimentation and has implemented the Care Culture Plan , reinforcing its ethical and social commitment to animal welfare and the staff who work with them.
Documentation and regulations
- Internal regulations of the CEEA ibs.GRANADA
- Guide to carrying out a research project with animals in the animal facility
- Royal Decree 53/2013, of February 1, which establishes the basic rules applicable to the protection of animals used in experimentation and other scientific purposes, including teaching.
- Royal Decree 1083/2025, of December 2, amending Royal Decree 53/2013
- Order ECC/566/2015, of March 20, establishing the training requirements that must be met by personnel who handle animals used, bred or supplied for experimentation and other scientific purposes, including teaching.
The Platform is located in the basement of the Licinio de la Fuente Peripheral Specialty Center, attached to the Virgen de las Nieves University Hospital, and has a total area of 907 m².
General services
- Housing and care of the animals (feeding, water, bedding and environmental enrichment)
- Consulting on well-being, management, and experimental design
- Quarantine and health control according to FELASA criteria
- Veterinary support
- Sterilization of material
- Changing rooms and access control
- Operating room services, anesthesia and post-operative care
- Organization of courses and training activities
Laboratory services
- DNA and RNA purification
- conventional and real-time PCR
- Microarrays
- Immunoassays (ELISA, Western Blot)
- Automated serum and hormonal biochemistry
- Ultracentrifugation and molecular analysis
specific services
- LIC 01 - Micromethod of Animal and Human Experimentation
- LIC 02 - Isolation of blood components
- LIC 03 - Isolation of Mononuclear Cells
- LIC 04 - Determination of Creatinine in urine samples
- LIC 05 - Determination of lipid profile in rodent plasma samples
- LIC 06 - Extraction and quantification of cortisol levels in hair samples
- LIC 07 - Cortisol extraction in saliva samples
Animal area
- Quarantine/isolation room
- Rat Room
- Mouse room
- Room for immunocompromised rodents
- Rabbit room
- Pig room
- Experimental room/laboratory
- Clean material warehouse
- SPF material zone
- Wash zone
Surgical area
- Two experimental operating rooms
- Pre-operative room
- Warehouse supplies and pharmacy
General and support laboratory area
- Basic molecular biology laboratory room
- Positive pressure cell culture room
- Cryopreservation room
- Bioanalyzer Room
- Conventional rodent racks
- Racks with interconnectable cages for rabbits
- Ventilated cabinets for immunocompromised rodents
- Individual identification system using injectable microchips
- Thermal washer-disinfector for cages and other accessories (Easy 400, IWT, Tecniplast)
- SAS modular cabin (Matachana)
- Vertical autoclaves (Raypa)
- CO2 euthanasia team
- Small equipment for performing basic techniques with experimental animals (probes, clippers, etc.)
- Surgical instruments for laparoscopy, traumatology, urology, otolaryngology and microsurgery
- Surgical aspirators
- Infusion pumps
- Defibrillator
- Electrocoagulators
- Ligasure Maryland Sealant/Divider (Medtronic)
- Laparoscopy towers
- Inhalational anesthesia equipment (sevoflurane)
- Magnifying glasses
- Zeiss TIVATO-700 Surgical Microscope
- OPMI 1 surgical microscope, Zeiss
- Zeiss OPM 212F Surgical Microscope
- CO2 Insufflator
- Vortices, magnetic stirrers, precision balances, centrifuges, pH meter, ultrasonic bath, etc.
- Microarray scanner analyzer
- Automatic analyzers
- Thermostatic baths
- Vertical laminar flow cabinets (Faster)
- Refrigerated ultracentrifuge (Avanti J-30I Beckman coulter)
- Cryostat (Leica)
- Hybridization oven
- Shaking incubator
- CO2 Incubators
- Motic AE21 Inverted Microscope
- Inverted fluorescence microscope (Zeiss)
- Tissue homogenizer
- Thermal cyclers
- Vacuum pump
- cobas c-311 analyzer (ROCHE)
- cobas e-411 analyzer (ROCHE)
