Cell Culture Platform
This unit offers its users (self-service mode) the possibility of cultivating and experimenting with all types of eukaryotic cells, both primary cultures and established cell lines. In addition to providing scientific advice to users, the possibility of carrying out a wide variety of techniques is also facilitated, which allows the study and detailed characterization of cell cultures, both at the molecular and cellular level, with the support of the corresponding Units of Flow Cytometry, Microscopy and Molecular Imaging, and Genomics.
This platform has a portfolio of services mainly related to the application of different techniques for the detection of the hormonal activity of pure compounds or mixtures of compounds of different origin, not available in other institutions. This makes the platform´s services highly requested by personnel outside the Institute. In addition, the platform has an experimental irradiator for cell cultures.
It is located on the 1st Floor, Old Management Building, San Cecilio Hospital, Avda. Madrid sn de Granada. Surface: 41.83 m2
There are two modes of service provision:
- Self-service: use of the cell culture room for days. The cost is specified in the document “Rates”, which is available to users on the ibs.GRANADA website.
- Performance of Specific Techniques by the Responsible Technician. The cost of these techniques depends on their complexity and the number of samples to be analyzed, and must be individually budgeted.
- CC_01-Test E-Screen: "in vitro" determination of estrogenic and/or anti-estrogenic hormonal activity by means of cell proliferation techniques
- CC_02-Determination of androgenic and/or anti-androgenic activity by in vitro techniques for modulating gene expression in prostate cancer cells
- CC_03-MELN reporter gene assay: quantification of estrogenic and/or anti-estrogenic activity in breast cancer cells using in vitro gene expression techniques
- CC_04-Androgenic activity in MCF-7 AR1 breast cancer cells using in vitro cell proliferation techniques
- CC_05-Obtaining steroid-free serum by carbon-dextran treatment
- CC_06-Maintenance of tumor cell lines in culture
- CC_07-Obtaining transfected cell lines "vitro" using modular electroporation techniques
- CC_08-Determination of viability/cytotoxicity in eukaryotic cells from monolayer cultures
- CC_09-Training/Training of personnel in “in vitro” cell culture techniques for eukaryotic cells
- CC_10-Rapid detection of mycoplasmas in cell cultures
- CC_11- Double determination of “in vitro” hormonal activity against the human estrogen receptor alpha (hERα) using different tumor cell lines of mammary origin
- Biological safety hoods:
- Telstar Bio II A / M (2 units).
- Cultair BC 100.
- Gelaire Class 100.
- Sanyo MCO-20 AIC CO2 incubators for cell culture (2 units).
- OLYMPUS IX53 inverted microscope.
- Leica M2 inverted fluorescence microscope.
- Centrifuges:
- EPPENDORF 5810R centrifuge, tilting rotor for 15 and 50 ml tubes.
- Microcentrifuge EPPENDORF 5424.
- Microcentrifuge EPPENDORF 5415R.
- Microcentrifuge Orto Alresa Biocen 22R.
- Centrifuge Orto Alresa Digicen 20R, with rotor for plates.
- Eppendorf ultracentrifuge.
- Memmert WNE 14 thermostatted water bath.
- Precision Electronic Balance GR 202 with double weighing range.
- Plate Reader (Photometer, luminometer and fluorimeter) Dynex Technologies TRIAD.
- Heildolph unimax 1010 shaking incubator.
- BIO-RAD TC20 Automated Eukaryotic Cell Counter.
- Gene pulser Xcell electroporator (Modular electroporation system for the transfer of all types of cells).
- Fridge and freezer.
